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Measurements of lysosomal accumulation and retention of foreign chemicals have proven to be easy means to obtain data on the health status of cells, and on the level of expression of multixenobiotic resistance transporter proteins Both of these are prognostic for more debilitating effects of prolonged and heavier exposures to toxic chemicals (Bayne & Moore, 1997) Additional assays which have been used productively in environmental toxicology with these animals include measurements of induced metal-binding proteins (metallothioneins), induced enzymes with oxygenscavenging activities (superoxide dismutase, catalase) and metabolising activities for polychlorinated hydrocarbons (cytochromes P450), phagocytosis, respiratory burst and the plasma concentrations of various humoral factors (see Bayne & Moore, 1997) In the speci c context of molluscan blood cells, these latter are generally rich in lysosomes, phagocytically active and highly responsive to pollutant chemicals (Grundy et al, 1996a,b; Lowe et al, 1995a,b; Moore et al, 1996a,b; Viarengo et al.

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frequently lack the nonobviousness required of a utility patent. Moreover, the current pace of technological advancement is such that chips are often obsolete within two years less than the average period of time to process a patent application in the Patent Office. To provide intellectual property protection under these trying conditions, Congress passed, in 1984, the Semiconductor Chip Protection Act, creating a new form of intellectual property (actually one closely related to a copyright, with a few patent-like aspects). The act prohibits copying of original mask works that have some degree of originality they cannot be mere commonplace variations of previous designs (this is one of the aspects borrowed from patent law). Registration is very similar to copyright registration and is, in fact, administered by the Copyright Office. Protection is effective upon registration or commercial exploitation (first sale, offer for sale, or other distribution to the public), whichever occurs first. However, such protection terminates two years after exploitation has begun, unless an application for protection has been filed. If registered, protection runs for 10 years from the time it began. Although it is not required, the owner of a mask work may affix a notice (comprising the words mask work, the symbol M or the letter M in a circle, and the name or designation of the owner) on packaging for products employing the mask work, thereby giving constructive notice of its protected status.

, 1994; Winston et al, 1996) Lysosomes form an important part of the haemocyte s physiological apparatus, for it is in the lysosomal compartment that foreign cells are killed and degraded to monomeric chemical constituents (ie, cell feeding) Consequently, any pollutant-induced damage to lysosomal function will impact directly on the cellular immune process which is dependent on effective phagocytic engulfment of invading organisms or abnormal self followed by intracellular digestion/degradation In fact, such effects have been clearly demonstrated both in vitro and in vivo in the haemocytes of the marine mussel (Mytilus edulis) by Grundy et al (1996a,b)..

Electrode Reactions of Dissolved Oxygen, Dissolved Hydrogen, Carbon Dioxide and Solvated Electrons Dissolved Oxygen The solubility of oxygen at 1 atm and 25 8C has been determined to be 1.0 mM in water, 2.1 mM in DMSO, 4.8 mM in DMF, 8.1 mM in AN, * 10 mM in hydrocarbons, and * 25 mM in fluorocarbons [39 a]. In aqueous solutions, the first reduction step of the dissolved oxygen is either a two-electron or four-electron process, depending on the catalytic activity of the electrode material and on the solution composition:

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